Abstract
Summary
Skin fibroblasts from normal individuals were grown in Ham's F-10 and Eagle's minimum essential medium and the intracellular concentration of amino acids measured using an automatic amino acid analyzer. Cultivated human skin fibroblasts exhibited a characteristic pattern of free amino acid content which differs from the amino acid pool previously described in Hela and L cells as well as many mammalian tissues and biological fluids. The free amino acid pool of fibroblasts grown in Eagle's MEM nutrient mixture was generally larger than that found in cells grown in Ham's F-10 medium. However, the contribution of most amino acids to the pool was comparable using both media. These data suggest that the measurement of the free amino acid content of cultivated human skin fibroblasts may prove useful for the study of inborn errors of amino acid metabolism in cell culture.
Get full access to this article
View all access options for this article.