Abstract
Summary
A method of purification, which employs Ficoll gradients and rate-zonal centrifugation, is described which rapidly separates avian myeloblastosis virus in high-titer viremic plasma from material that is associated with the process of intracardiac exsanguination of leukemic chickens. A nearly threefold increase in specific activity of adenosine triphosphatase (ATPase, an enzyme of the viral envelope) is achieved during purification. Ribosomal-like species of RNA of 18S and 28S are found in AMV purified by this procedure. In addition, a simple method based on viral turbidity is available for approximate quantitation of this purified AMV in terms of viral protein and ATPase activity.
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