Abstract
Summary
The SM strain of feline sarcoma virus (SM-FSV) isolated by in vivo methods from a naturally occurring case of feline fibrosarcoma was characterized in vitro. The virus propagated and induced morphologic transformation of homologous feline host cells as well as heterologous host cells of human, canine and porcine origin. The virus propagated in feline cultures retained its oncogenic potential for newborn cats.
The SM-FSV was found to contain an associated nontransforming feline C-type virus at a concentration of approximately ten fold higher level than the cell transforming virus. The sarcoma virus transformed feline cells with one-hit kinetics suggesting that this associated nontransforming virus is not required for the initiation of the cell transforming event.
Viral interference tests and viral neutralization tests with type-specific antisera suggested that the SM-FSV consisted of a mixture of antigenic types of the feline leukemia-sarcoma virus subgroups A and B. Isolation of a A subgroup nontransforming virus from virus stocks of the nontransforming SM-FSV associated virus was achieved by virus cloning techniques.
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