Abstract
Summary
Renal adenocarcinomas can be induced in Rana pipiens with equal success after injection of virus containing cell fractions derived from either the cytoplasmic or nuclear portion of renal tumor cells with intranuclear inclusions. The latter result supports the contention that a nuclear herpes-type virus (HTV) is implicated in tumor induction. Brief periods of sonication do not alter the oncogenicity of either cell fraction. Pretreatment of the viral cell fractions with a proteolytic enzyme or lyophilization of the fractions prior to injection destroys all oncogenic action. Postinjection heat shock of the injected embryos also prevent the formation of renal tumors. Postinjection cold treatment of induced tumors results in formation of intranculear inclusions, an index of viral replication.
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