Abstract
Summary
The influence of various maneuvers designed to alter energy metabolism on spontaneous and induced release of ACTH from isolated pituitary cells was studied. Spontaneous release was increased by cold (2°) but not significantly changed by anoxia, glucose removal, DNP, iodoacetate, oligomycin, or antimycin A. On the other hand, induced release of ACTH was inhibited by all of the maneuvers tested. These findings indicate that induced release of ACTH from isolated pituitary cells is an energy dependent process.
The author is grateful to Dr. George Sayers for his invaluable advice during the conduct of these experiments and preparation of the manuscript and to Beth Portanova and Marshall Clark for their technical assistance.
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