Buoyant Density of Bovine Viral Diarrhea Virus

Abstract

Summary

Bovine viral diarrhea virus (BV DV), strain NADL, was purified by removing extracellular fluid from infected cultures at 96 hr postinoculation and clarifying by low speed centrifugation. Clarified fluid was concentrated by centrifugation onto a two-step 20-50% sucrose discontinuous gradient. Most of the infectivity was associated with a light scattering band in the region of 20% sucrose. ³H-Uridine labeled and unlabeled viral band material was then pooled, diluted, and isopycnically banded through a sucrose density gradient. Infectious BVDV was recovered from fractions between 1.075 and 1.115 g/cm³. The main peak of infectivity and radioactivity was found at a density of 1.092 g/cm³.