Abstract
Summary
Three purified Parvoviruses (H-1, H-3 and RV), treated with various proteolytic enzymes, showed chemically-induced changes in their hemagglutination pattern only after exposure to papain which enhanced the values found. This increase was probably due to a dual effect of the papain: one on the virus itself by removing bound contaminants and, two, on the red cell membrane, probably by reducing the negative charge. Although the HA titer of virus was altered by papain treatment, no change in the SDS-acrylamide electrophoretic pattern of the capsid proteins or in infectivity was observed.
The authors thank Miss Barbara Madden for technical assistance.
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