Effect of 2-Acetoamidoethyl ( p -Chlorophenyl) ( m -Trifluoromethylphenoxy) Acetate (Halofenate) on Cholesterol Oxidation by Rat Liver Mitochondria 1

Abstract

Summary

We have compared the effects of halofenate [2-acetoamidoethyl (p-chlorophenyl) (m-trifluoromethylphenoxy) acetate] and clofibrate (ethyl p-chlorophenoxyisobutyrate) on rat serum and liver lipids and on oxidation of cholesterol by suitably fortified preparations of rat liver mitochondria. The halofenate was fed as 0.05% of the diet and the clofibrate as 0.3%. Both compounds caused increases in liver size and liver lipids. In the 3-week feeding experiment, both test compounds showed a significant hypocholesteremid effect.

When complete incubation mixtures were used, the oxidation of 26-¹⁴C-cholesterol to ¹⁴CO₂ was no different for liver mitochondrial preparations from halofenate, clofibrate, or control rats. However, in the absence of boiled supernatant factor (cytosol), oxidation of cholesterol was significantly higher with liver mitochondrial preparations from the drug-treated rats.