Selective Collection of Granulocytes 1

Abstract

Conclusions and Summary

Glass wall attachment can be used as a method of concentrating granulocytes. Enrichment of granulocytes can be calculated from the data given in Table I and is shown in Fig. 5. The enrichment is shown to be dependent on total cell concentration and to increase with a decrease in cells/ml in the culture.

Under the given condition of a 5-ml-incubation volume and a glass surface of 46 cm², quantitative granulocyte enrichment of over 95% is possible if the total cell concentration is under 5 × 10⁶/ml. The specificity of granulocytic attachment decreases with an increase in cell concentration, perhaps because an increase in lymphocytes interferes with granulocytic attachment. One centimeter square of glass wall can be saturated with 1.7 × 10⁶ granulocytes.

The author wishes to thank Tim Oren for his assistance in revising the manuscript.