On the Biological and Chemical Reactivity of Carbamylated Glucagon 1

Abstract

Summary

Glucagon was carbamylated with carbamyl phosphate and with KCNO. Only the NH₂-terminal histidine was carbamylated. Carbamylation of glucagon resulted in loss of its adipokinetic and hyperglycemic effects in the intact duck, for the two forms of carbamylation used. These results indicate that the N-terminal histidine of glucagon is essential for the biological activity of this hormone.

We thank Dr. J. Kimmel for the amino acid analysis and Mrs. Alice Diederich and Miss Donna E. Swenson for excellent assistance.