Abstract
Summary
Data are presented supporting the use of induction of ovulation in CPZ-blocked rats as a suitable biological assay for FSH. The assay is sensitive over a dose range of 10-28 μg of FSH compared to a dose range of 30-160 μg for the conventionally accepted bioassay of Steelman and Pohley. The assay is made specific for FSH by enzymatic digestion of any LH contaminant. Results obtained with this type of assay demonstrated on different days of the estroiis cycle pituitary fluctuations in FSH concentration paralleling those reported using the conventional Steelman-Pohley assay.
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