Abstract
Summary
Gel filtration can be used to fractionate LH125I into three components: aggregate, monomer, and subunit. Only the monomer has satisfactory immunoreactivity. The plot of logit 100 B/B o vs nanograms unlabeled hormone is linear over the range of the standard curve. The slope deviated from the theoretical value of —2.303, probably because of the use of an excess amount of specific antiserum. The high resolution gel filtration used provides an assessment of the quality of the LH125I on the day of preparation, and can also be used over a period of 7-8 weeks to isolate sufficient immunoreactive monomer from the heterogeneous stock solution to perform radioimmunoassays.
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