Abstract
Summary
Successful colonization of lipid-laden cells were obtained from diluted single cell suspensions prepared from atherosclerotic lesions of cholesterol-fed rabbits using col-lagenase digestion followed by incubation in medium F12 and Eagle's minimum essential medium supplemented with equal proportions of rabbit and fetal bovine serum. Colonies of endothelioid cells containing Sudan black B staining particles appeared similar to foam cells in the rabbit aortic intima by electron microscopy.
The growth in culture of colonies of lipid-laden cells opens new possibilities for controlled cell studies on the origin of foam cells in atherosclerotic lesions.
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