Abstract
Summary
A method of analyzing trace serum proteins is presented which involves acid methanol precipitation of serum proteins, extraction in a Soxhlet with chloroform and methanol, and electrophoresis of the redissolved proteins in acid-urea starch gels. Relatively few bands are obtained which are well separated and discrete. We have presented evidence that two of the bands represent the fourth component of complement, C4, and a more rapidly migrating band is a conversion product of C4. We were unable, after analyzing 143 randomly collected fresh sera and sera from 65 members of 14 families, to establish a pattern of autosomal codominant inheritance at a “C4 locus.”
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