Abstract
Summary
A sensitive and reliable system to culture mouse lymphocytes stimulated to transform by phytohemagglutinin is described. This system permits the convenient use of a relatively rapid modified “acidinsoluble” method to assay nucleoside incorporation. Under the conditions described, stimulated splenic and peripheral lymphocytes demonstrated elevated RNA synthesis 18-24 hr prior to maximum DNA synthesis. Nucleoside incorporation of stimulated peripheral lymphocytes exceeded that of splenic cells and occurred earlier. The degree of transformation was dependent on cell density, phytohemagglutinin-P concentration, and serum level.
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