Abstract
Summary
X-Irradiation of microsomal particles prepared from pig thyroid homogenates by conventional methods with 100, 000 rads before incubation with radioactive leucine reduced protein synthesis as much as 60% of that of a nonirradiated control. The effect of the radiation was most pronounced at early incubation times (15-30 min) and at low concentrations of the supernatant fraction. β-Mercaptoethanol, which was necessary for protein synthesis, reduced the amount of X-ray damage to protein synthesis considerably when added before X-irradiation, but had minimal effect when added after X-irradiation.
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