Abstract
Summary
Antisera to various purified preparations of the lymphocyte stimulant PHA have been prepared in rabbits. These antisera neutralized lymphocyte activation by PHA but were not toxic. Using such antisera, we were not able to detect any differences between the mitogenic components of different purified preparations of PHA, including the separated erythroagglutinating and nonerythroagglutinating fractions. Antisera prepared against purified fractions of PHA also inhibited the mitogenic effects of crude PHA extracts. These antisera also prevented lymphocyte activation by the closely related wax bean hemagglutinin but did not inhibit stimulation by concanavalin A, lentil extract, pokeweed mitogen, staphylococcal filtrate, or antithymocyte serum.
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