Abstract
Conclusions and Summary
The presence of constant low levels of interferon in the growth medium does not affect the ability of primary human foreskin cells to establish themselves in tissue culture. In addition, continuous exposure of human foreskin fibroblasts to up to 12 units/ml of human interferon does not alter the limited lifespan of these cells in vitro (7). Cells continuously exposed to these levels of interferon are still able to respond normally and in a linear fashion to dilutions of interferon by becoming resistant to virus challenge.
No change in the ability to produce interferon in response to poly I:C was noted in continuous-interferon cell lines. Production of interferon in response to NDV challenge was unaltered by cells exposed to a constant 5 units/ml of interferon, but exposure to 12 units/ml caused moderately reduced yields.
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