Abstract
Summary
It was shown that canine prostatic fluid (CPF) could be fractionated into three components by gel filtration on Sephadex G-100. These components were referred to as pools 1, 2, and 3 in order of elution and their relative proportions were 1, 9, and 90%, respectively, of the total recovered protein. The sedimentation coefficients for pools 2 and 3 were 4.0 S and 2.6 S, respectively. Whole CPF, on the other hand, showed only one peak of 2.5 S.
Antigenic analysis of whole CPF, in gel diffusion, showed three lines of precipitation when tested against rabbit anti-CPF. Reactions of pools 2 and 3 consisted of two lines each; one faint one and the other more intense and sharp. The more intense line of precipitation of pool 2 was inhibited by a component of pool 3. It was suggested that pool 3 might contain low molecular weight substance(s) with antigenic sites similar to those on the main antigen of pool 2.
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