Abstract
Summary
Mannide monooleate and mannide dioleate fractions were obtained by vacuum distillation and column chromatography of Arlacel A. Different densities, stabilities, and different toxicities were found. The monooleate fraction was readily hydrolyzed by pancreatic lipase with consequent release of free oleic acid and it exhibited also severe tissue toxicity when injected intradermally into guinea pigs. In contrast, the dioleate fraction was not so susceptible to hydrolysis and showed minimal effects when injected intradermally. The findings support the hypothesis that the toxic effect of certain batches of Arlacel A is due to release of free oleic acid in vivo.
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