Abstract
Summary
Sucrose gradient analysis of Venezuelan equine encephalitis virus, a group A arbovirus, indicated that hemagglutinating (HA) and complement-fixing (CF) activities were coincident with virus infectivity. In contrast, West Nile, Ilheus, and Rio Bravo viruses of group B were fractionated into infectious and noninfectious components. The infectious component sedimented as a single entity, possessed HA and CF activity, and had a buoyant density of 1.19 g/cc. West Nile and Ilheus virus suspensions contained a slower sedimenting noninfectious HA and CF component with a buoyant density of 1.16 g/cc. Rio Bravo virus suspensions did not contain a similar component. A very slow-sedimenting noninfectious CF component (6 S, 1.10-1.12 g/cc) was detected for each of the group B viruses.
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