Growth of Parainfluenza Virus 1 in Isolated Perfused Rat Lungs 1

Abstract

Summary

Isolated, homolateral lungs of cesarean-derived barrier-sustained rats were maintained by a vascular perfusion with oxygenated tissue culture medium and supported the growth of the Sendai strain of parainfluenza type 1 virus.

Production of hemagglutinin by perfused lungs was greater than production by comparable quantities of sliced lung maintained in oxygenated perfusion medium. Homolateral lungs were required for controlled experiments of hemagglutinin production in perfused lungs. Pulsatile flow of perfusion fluid led to production of higher titers of hemagglutinin than flow at a constant rate. No differences were found when 5% CO₂/95% O₂ was compared with 5% CO₂/95% air and increase in virus production was not noted with several variations of the perfused medium.