Abstract
Summary
The efficiency of plaquing (EOP) on chick embryo fibroblast monolayers of three temperature-sensitive mutants of Sindbis virus defective in late functions was enhanced 10- to 11-fold when the inoculum was applied in medium containing 66% of the normal concentration of sodium chloride. Studies with one of these mutants revealed similar enhancement over a broad range of reduced salt concentrations. The EOP of wild-type virus and of a temperature-sensitive mutant defective in RNA synthesis were also enhanced, but to a lesser degree. No increase in EOP occurred if the exposure of virus to reduced salt concentration ended before the virus suspension was applied to the monolayers; thus, a virus-cell interaction appeared to be involved. The salt-sensitive step occurred within the first hr of infection, but subsequent to virus adsorption.
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