Abstract
Summary
The inhibition of the migration of sensitized peritoneal macrophages has been used in studying a specific tumor transplantation antigen in Morris hepatoma 5123 and its absence in normal liver tissue from which the tumor originated.
Migration of peritoneal macrophages sensitive to the tumor was greatly inhibited by the addition into the incubation medium of a fraction of the solubilized tumor cell membranes. Use of solubilized membranes from normal liver cells were much less effective in inhibiting the migration of sensitized peritoneal macrophages.
The specificity of the inhibition was demonstrated by using decreasing concentrations of the tested antigens. By this method it was possible to reach a point where the test was reflecting only the tumor-specific activity.
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