The Influence of Thyrotropin on Harderian Gland Glycosaminoglycans of the Mouse 1

Abstract

Summary

The nature of ³⁵S sulfate accumulated by the harderian gland of untreated and thyrotropin-treated mice was characterized by Sephadex G-25 and cellulose-column chromatography. Most of the ³⁵S remained inorganic but some was organically incorporated; a single injection of 10 mU thyrotropin increased the radiosulfate content of both moieties and higher doses of thyrotropin had no greater effect. Salivary gland and pancreas showed similar trends of response to thyrotropin. In mice pretreated with thyroid hormone, thyrotropin (as reported previously) increased ³⁵S incorporation into sulfated glycosaminoglycans, especially keratan sulfate and dermatan sulfate, in harderian gland; under these conditions there was also an increase in total uronic acid content.