Abstract
Summary
Hemagglutinin of titer 1:384 or more could be produced regularly in continuous hamster embryonic cell culture 2 to 4 days after injection with JEV, provided that the culture was maintained with serum-free medium containing bovine or human albumin. The cell culture HA was stable for more than 3 months at 4° and 1 year at —60°.
Titers of cell culture HA were highest when phosphate buffer salines of pH 7.3 and 4.3 were used as diluents for HA and red blood cells, respectively. The optimal pH of the HA-red cell mixture was about 6.5. Goose red cells yielded better results than cells from day-old chicks or roosters.
Two hundred and forty-four sera from monkeys and pigs were tested in parallel with both cell culture and mouse brain HA for HAI antibody. There was a high degree of correlation between the titers obtained (0.95).
Replacement of traditional mouse brain HA by cell culture antigen in the HAI test of JEV infection is recommended.
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