Base Composition and Molecular Weight of DNA from a Frog Polyhedral Cytoplasmic Deoxyribovirus 1

Abstract

Summary

Frog PCDV DNA was estimated from its melting profile to contain 54–55% G + C. The molecular weight of PCDV DNA was confirmed to be about 130 × 10⁶ daltons from its sedimentation behavior in neutral sucrose and by contour length measurements. The mean content of DNA per PCDV virion in two preparations of purified virus was 1.86 × 10^-16 g (112 × 10⁶ daltons) and 2.56 × 10^-16 g (154 × 10⁶ daltons). Data suggest that PCDV virions contain a single linear double-stranded molecule of DNA.