Abstract
Summary
Cell sap must be passed through Sephadex G-25 and then dialyzed to remove all of the amino acids. The cell-free system for protein synthesis has an optimal amino acid requirement which is approximately the same level as that observed in normal cell sap and the absence or excess of amino acids can markedly inhibit the rate of protein synthesis in the ribosomal-dependent system. In the nonribosomal system, the incorporation rate as well as the amino acid requirement are low.
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