Abstract
Summary
Two established cell lines, derived from the embryonic tissues of chinook salmon and steelhead trout, respectively, have been examined for their ability to support the replication of two viruses of salmonid fish, and two viruses of warm-blooded vertebrates. The sockeye salmon virus (Oregon strain) produced large yields of progeny virus upon primary inoculation in the steelhead trout cells, and was readily propagated through five serial transfers. Infection was accompanied by a characteristic cytopathic effect on the cells. In the chinook salmon cells, replication of the virus was limited upon primary inoculation, but was greatly enhanced after a series of five culture passages. The Sacramento River chinook virus was also found to replicate in both of these cell lines upon primary inoculation, and the agent was transmitted through five serial passages. The cytopathic effect produced by this virus appeared essentially identical to that of the sockeye salmon virus. It was found that both salmonid cell lines would support replication of the western equine encephalitis virus very adequately at 26°. At 23° replication of the virus was very limited in the chinook salmon cells and none could be detected in the steelhead cells at the end of a 7-day incubation period. The virus was propagated through five serial transfers in both cell lines, with the production of a marked cytopathic effect. The virus of Newcastle disease failed to replicate in either cell line.
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