Quantitative Biological Assay of Bacterial Endotoxins 1

Abstract

Summary

The administration of AM-D simultaneously with typhoid endotoxin to mice reduced the 7-day LD₅₀ of the latter approximately 550 to 20,000-fold. This system was used to quantitate the endotoxin contents of minute amounts of purified meningococcal LPS as well as broth culture super nates and cell bodies of these organisms. Small quantities of endotoxin were detected in one meningococcal polysaccharide preparation but not in another which was nonpyrogenic in rabbits.