Abstract
Summary
The production of adenovirus type 7 T-antigen(s) and virion antigens in infected KB cells has been detected by immunofluorescence and correlated with the one-step growth curve. The T-antigen first appears at 4 hr as small discrete intranuclear dots and flecks which extend with time to form stellate configurations and eventually long branching filaments by 12 hr after infection. Virion antigens appear at 9-10 hr and new infectious virus is detected by 12 hr. The appearance and morphology of the T-antigen is unaffected by the presence of FUDR; however, the synthesis of virion antigens is delayed until about 7 hr after reversal of the inhibition with thymidine. The growth kinetics of adenovirus 7, a member of the weakly oncogenic group of human adenoviruses, are similar to those of the “nononcogenic” types 2 and 5 in contrast to the longer time required for the growth of the highly oncogenic adenovirus 12.
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