Abstract
Summary
Under Ionagar overlay medium, the prototype strain of rhinovirus type 34 was found to consist of a mixture of two plaque types designated m (minute) and m + (large). Large plaques were obtained with m virus when agarose was used or when polycations (protamine or DEAE-dextran) were incorporated into Ionagar medium. Starch gel medium completely inhibited the appearance or m but not m + plaques. However, the m virus formed plaques under starch when polycations were incorporated. The nature of the inhibitory principle in starch is unknown. The pH of overlay media did not appear to influence the plaque size of either mutant.
Temperature of incubation had little if any effect on replication of m virus. At 37°, the m + mutant required an adaptation period of three to four cell culture passages before maximum yields of infectious virus were obtained.
No differences between the mutants were observed in buoyant density, rate of adsorption, host cell tropism, or antibody sensitivity.
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