Abstract
Summary
IgM was prepared from normal rabbit serum and from the serum of a human patient (Aug.) suffering from Waldenström's macroglobulinemia. In each of the preparations, there were one or more components which sedimented in the analytical ultracentrifuge much faster than IgM. Such large components found in preparations of human IgM have been attributed in the past to both covalently and noncovalently associated polymers of IgM. Studies with the rabbit system have not previously been done. It was found that most of the faster-sedimenting materials in the preparations of human IgM, and all of that in preparations of rabbit IgM, dissociated to IgM size in 4 M or 6.7 M guanidine-HCl solutions, pH 7. It was concluded that all of the faster-sedimenting material accompanying the rabbit IgM, and most of that attending the human IgM, was an aggregation of the respective IgM held by noncovalent bonds.
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