Biosynthesis of Liver Microsomal Phosphatidyl Cholines During the Development of Choline Deficiency 1

Abstract

Summary

The incorporation of 1,2-¹⁴C-choline and 1,2-¹⁴C-ethanolamine into the total phospholipid-P, total lecithin P, and the phosphatidyl choline-P fractions of liver microsomes at 1, 2, 3, and 4 hr after intraperitoneal injection of the isotopic compounds in choline-deficient animals was studied. The incorporation of 1,2-¹⁴C-choline into the total phospholipid P and lecithin P of liver microsomes was greater in the choline-deficient than in the control animals. The greatest incorporation occurred in lecithin fractions 3 and 4 in the choline-deficient animals. The greatest incorporation of 1,2-¹⁴C-ethanolamine was in the lecithin fractions 1 and 2 in both controls and choline-deficient animals. There was a decrease in the incorporation of 1,2-¹⁴C-ethanolamine into the lecithin fractions of microsomes from the choline-deficient animals.