Abstract
Summary
For recovery of measles virus from brain tissue dissociation by trypsinization was superior to cell isolation by mechanical and chemical treatment. Optimal techniques for dispersion of brain cells were established. When tested in cell cultures or in animals dissociated cells transferred infectivity much more effectively than did brain homogenates. Isolation of measles virus was successful up to 18 hr after the death of animals in tissue culture and up to 22 hr when assayed in suckling hamsters.
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