Mouse Hemoglobin from Bone Marrow,Spleen,and Peripheral Blood Cells 1

Abstract

Summary

Upon fractionation by starch-gel electrophoresis:

(1) In the mouse with the single-type hemoglobin (C57BL/Cum) or the mouse with the β chain component being from the single-electrophoretic benzidine-positive fraction of the same mobility as that of hemoglobin from the peripheral blood was found in cell hemolyzates of bone marrow and spleen,

(2) In the mouse with diffuse type hemoglobin (Tenn-Swiss) or with the β chain component from the diffuse type of hemoglobin (α^C57BL/Cumβ^BALB/c), previously reported four hemoglobin fractions were seen in the peripheral blood; however, only two benzidine-positive fractions were found in the spleen and bone marrow.

Phenylhydrazine-induced anemia is accompanied by changes in the electrophoretically fractionated benzidine-positive materials from spleen and bone marrow and in the hemoglobin fractions from the peripheral blood.