Abstract
Summary
When bone marrow stem cells are assayed simultaneously by the spleen colony technique and the in vitro agar system following treatment of donors with hydroxyurea a differential effect was observed. There was found to be approximately a 50% reduction in the number of bone marrow in vitro colony forming cells after DNA synthesis inhibition by hydroxyurea and only a slight change in the pluripotential stem cell compartment by the spleen colony technique.
It appears therefore that the in vitro colony forming cell derives from a proliferating stem cell compartment, probably myeloid committed, and is distinct from the nonproliferating pluripotential transplantable stem cell.
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