Abstract
Summary
Most rabbits treated topically with 2,4-dinitrofluorobenzene produced circulating precipitins and passive hemagglutinins. Animals sensitized by a primary painting series and re-exposed topically to DNFB all demonstrated anamnestic antibody response. The time required for a peak titer to be reached after a second contact with the chemical was approximately one-half of that following the first application series. The relative titers following a skin test versus a second painting series suggested that the magnitude of the anamnestic response may have been dose dependent. The antibodies produced by this treatment were apparently directed only against the dinitrophenyl group. No precipitating antibodies against the autologous carrier were detected. No correlation was seen between the amount of precipitating antibody present and the intensity of the skin test. The development of contact hypersensitivity to a simple chemical compound in the rabbit was shown.
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