Abstract
Summary
In an attempt to clarify the mechanism by which biological effects of the polynucleotide complex, I:C, are enhanced by the polycation, DEAE-D, the dose response of the polycation on cell protection and on the induction of interferon was determined for various concentrations of I:C Utilizing kinetic studies at 37 and 4° it was possible to show that attachment of I:C to cells is depressed by DEAE-D. In addition, as much as 100 μg/ml of DEAE-D did not inhibit the function of pancreatic RNase in digesting attached I:C. Pretreatment of cells alone before I:C attachment, gave definite, although reduced enhancement of I:C function, and this enhancement could be negated with the polyanion heparin.
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