The Effective Molecular Titration of the Early Components of Dog Complement 1

Abstract

Summary

Effective molecular titrations of C1, C4, and C2 revealed values ranging from 75,000 to 123,000 for C1, from 70,000 to 228,000 for C4, and from 160 to 250 for C2 in the serum of five mongrel dogs. A striking feature of these experiments has been the efficiency of the homologous system as compared to that which employs guinea pig components. Complete or partial functional incompatibilities were demonstrated when: (i) C1^gp was employed to make EAC1 intermediate used for the titration of C4^dog; (ii) when EAC1^gp was used in the formation of the EAC4^dog cells which were converted to EAC14^dog cells for the titration of C2^dog; (iii) when C2^gp was introduced to prepare EAC14^dog cells for lysis by C-EDTA, and (iv) when lysis of EAC142^dog cells was attempted with C^gp-EDTA.