Abstract
Summary
Neoplastic transformation of rat embryo cells in vitro by Rauscher leukemia virus (RLV) is reported. Rat embryo cells infected initially with RLV have replicated infectious virus and produced complement-fixing (CF) antigen characteristic of the murine leukemia-sarcoma virus complex for more than 18 months. Cells from these cultures underwent neoplastic transformation in vitro and produced tumors when injected into homologous hosts. The tumors were serially transplantable, and subsequent transplants continued to carry the initial RLV and to yield CF antigen.
The authors wish to thank Mr. H. C. Turner, National Institutes of Health, for supervising CF tests, and Mrs. Ersell S. Richardson and Mrs. M. H. Joglekar for excellent technical assistance. We are also grateful to Dr. L. Rabstein, Microbiological Associates, Inc., for histological examination of tumors.
Get full access to this article
View all access options for this article.