Stimulation of Release and Synthesis of Growth Hormone (GH) in Tissue Cultures of Anterior Pituitaries in Response to GH-Releasing Hormone (GH-RH) 1

Abstract

Summary

Addition of small amounts of pure or highly purified porcine GH-RH twice daily to rat anterior pituitary tissue, maintained for 5 days in Trowell's T8 medium, significantly increased the amounts of GH in the medium and tissues relative to the controls as measured by bioassay. Apparent incorporation of radioactive amino acids into the GH bands on polyacrylamide gels after electrophoresis of medium and tissue was increased also. These results support other findings from this laboratory on the identity and properties of this neurohormone.

We are grateful to Mrs. C. Wooderson, Miss Nancy Moreland, and Miss K. Beightol for technical assistance and to the Endocrinology Study Section of the NIH for gifts of GH standard.