Attempts at Isolation of Lymphocytosis-Producing Factor from Supernatant Fluids of Bordetella pertussis Cultures 1

Abstract

Summary

The B. pertussis culture supernatant fluid possessing lymphocytosis-producing factor, histamine-sensitizing factor, lethal and hypoglycemia-producing factors was subjected to Sephadex G-10 and Bio Gel P-100 column chromatography. When chromatographic fractionation was carried out at 4° some fractions, found to retain the lym-phocytosis-producing property, had lost the lethal and hypoglycemia producing factors, and had partially lost the histaminesensitizing factor. Observations indicate that lymphocytosis-producing factor is distinct from other toxic factors.

We wish to acknowledge the technical assistance of Mr. Gerd Borner.