Abstract
Summary
A method for chromosome preparations from mouse peripheral lymphocytes is described. This method is based on the mobilization of lymphocytes from lymphoid tissues to peripheral blood by pertussis vaccine or by supernatant fluid from B. pertussis culture, on the effective separation of leukocytes from the erythrocytes by sedimentation with Plasmagel® and on the use of phytohemagglutinin (PHA) as mitogenic agent. Large numbers of well-spread metaphase plates suitable for detailed chromosome analysis can be obtained from a single mouse without sacrificing the animal. The procedure can be applied repeatedly and it was successful with all strains of mice tested.
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