Abstract
Summary
HeLa cells were separated into two portions by agglutination with anti-H ulex reagent, and the unreactive supernatant cells (2) were studied in comparison with the original cell suspensions (1). Examination of clones derived from these populations using fluorescent labeled anti-H reagent indicated that a high percentage derived from (1) reacted positively. On the other hand, those derived from (2) consisted of negatively reacting or mixed positive and negative clones. Subclones from these populations behaved in a fashion which characterized the parent cell colonies.
Note added in proof. The findings of Bottomley, Trainer and Griffin, J. Cell Biol. 41:806:1969 call attention to the importance of HeLa strain differences, since chromosome and biochemical differences were found among seven strains tested. Possible associations between karyotype and group H reactivity are currently being sought in this laboratory.
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