Isolation of Viruses from Primary Dog Cell Cultures and the Occurrence of Viral Antibody in Donor Animals

Abstract

Summary

Fifty-five different lots of primary dog kidney cell cultures and 30 lots of primary liver cell cultures (derived from stray dogs) were incubated for 4-5 weeks and examined for viruses. Ten ICHV's, ten CDV's, and one herpesvirus were isolated from kidney cultures; three ICHV's were isolated from liver cultures. Thirty-eight % of kidney cultures and 10% of liver cultures contained viruses. CDV was isolated only from dogs studied in early fall; no CDV was isolated from dogs studied in late winter and early spring. No characteristic antibody pattern was found in dogs yielding or not yielding viruses, since ICHV and CDV were isolated from dogs with and without antibody. Cell-free homogenates of CDV-infected kidneys were inadequate for demonstrating the virus, whereas primary cell cultures from these kidneys readily yielded virus. There was evidence from direct isolations and from antibody reactions that no adenovirus other than ICHV infected the dogs studied. ICHV antibody detection was about 60 times more sensitive by neutralization tests than by CF.