Abstract
Summary
An assay using the L-929 mouse fibroblast cell culture line was described to evaluate the toxicity of Arlacel A. The toxic lots of AA produced a characteristic cytotoxic effect consisting of intracytoplasmic refractile bodies. The results obtained by the cell culture and mouse assays were similar for 18 of 19 lots tested; one lot was moderately toxic by the cell culture assay only. A correlation was seen between the amount of FFA and the toxicity of AA. The cell culture assay provides a sensitive and reproducible assay to evaluate AA toxicity.
We thank Dr. B. S. Berlin for his critical review of the manuscript, the Cell Biology Section (DBS) for providing the cells used in these studies, and acknowledge the excellent assistance of Mr. Donald Riggs, Mrs. Norma Duffin, Mrs. Marion Grabowski, Mr. Ethan Summers, and Mrs. Hazel Young.
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