Abstract
Summary
Fourteen percent of guinea pig uteri survived freezing to a core temperature of —14° but none to —16°. Dose-response studies utilizing DMSO, dextran, and MgCl2 as cryoprotectants were conducted on guinea pig uteri frozen to —16°. Uteri treated with MgCl2 did not survive freezing. Uteri treated with dextran in concentrations as high as 20% survived freezing to —14° but not to —16°. The optimal dextran concentration, 10%, allowed a 62% survival at —14°. Uteri treated with 2 M DMSO survived freezing to —16°; and when treated with 5 M DMSO, survived cooling to —50°.
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