Effect of Stimulation on Pancreatic Amylase Secretion and Nuclear RNA Synthesis

Abstract

Summary

These studies were designed to obtain information concerning the temporal correlation of synthetic and secretory events in the pigeon pancreas after stimulation.

After methacholine administration secretion of amylase into the duodenum was apparent within 5 min, approached maximal rates within 20-30 min, and returned to basal levels by 60-80 min.

Feeding and methacholine administration were associated with enhanced uridine-³H incorporation into nuclear RNA by pancreatic tissue slices. The increased incorporation of uridine-³H into nuclear RNA after methacholine was apparent within 5 min, approached maximal rates within 20-30 min, and returned toward basal levels within 60 min. Methacholine activates processes resulting in secretion as well as synthesis. In these particular experiments the two processes were closely related in time.