Extraction of Plasminogen Activator from Canine Vascular Segments in Situ 1

Abstract

Summary

Plasminogen activator is easily recovered from canine veins in situ by solutions retained in vessel lumens, previously emptied of blood. The veins can be extracted repeatedly before they become exhausted. A pH of 5, low ion strength, and the presence of digitonon will enhance the plasminogen activator release. Low temperatures will block it. The external jugular vein generally yielded more plasminogen than did the femoral vein. The femoral artery yielded very little activator.